Live cell flattening - traditional and novel approaches.
PMC Biophys
; 3(1): 9, 2010 Apr 19.
Article
en En
| MEDLINE
| ID: mdl-20403171
ABSTRACT
Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method.PACS Codes 87.64.-t, 47.61.-k, 87.80.Ek.
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Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
PMC Biophys
Año:
2010
Tipo del documento:
Article
País de afiliación:
Alemania