DosR-regulon genes induction in Mycobacterium bovis BCG under aerobic conditions.
Tuberculosis (Edinb)
; 90(3): 197-200, 2010 May.
Article
en En
| MEDLINE
| ID: mdl-20421176
ABSTRACT
In this report we demonstrated that under aerobic conditions, Mycobacterium bovis BCG expressing an hsp60-driven second copy of the hypoxia-related transcriptional regulator DosR increased 2-fold or greater the expression of 38 out of the 48 genes belonging to the DosR regulon, including the latency antigens Rv1733c, Rv2029, Rv2627, and Rv2628. Expression of DosR under these conditions slightly delayed in vitro growth, but did not promote a non-replicating state as opposed to microaerobic and hypoxic adaptation. Our results suggest BCG producing DosR can be cultured under standard in vitro conditions, allowing evaluation of this strain as a latency-specific vaccine candidate.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tuberculosis
/
Vacuna BCG
/
Activación de Linfocitos
/
Regulación Bacteriana de la Expresión Génica
/
Regulón
/
Mycobacterium tuberculosis
Límite:
Humans
Idioma:
En
Revista:
Tuberculosis (Edinb)
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos