Rapid detection of immunoglobulin G against Mycobacterium tuberculosis antigens by two commercial ELISA kits.
Int J Tuberc Lung Dis
; 14(7): 841-6, 2010 Jul.
Article
en En
| MEDLINE
| ID: mdl-20550766
ABSTRACT
SETTING:
Tunisia.OBJECTIVE:
To assess the clinical usefulness of the commercial Pathozyme-Myco G (Myco G) and Pathozyme TB complex plus (Patho) enzyme-linked immunosorbent assay (ELISA) kits for the rapid diagnosis of active tuberculosis (TB) and to distinguish between active TB and non-TB pulmonary diseases in Tunisian patients.DESIGN:
Immunoglobulin G mediated humoral immune response against mycobacterial antigens (38 kDa and lipoarabinomannan, Myco G; 16 and 38 kDa, Patho) was evaluated in a group of active TB patients (128 smear-positive pulmonary and 33 extra-pulmonary samples) and in a control group (107 patients with non-tuberculous lung disease and two with leprosy). Active TB cases were confirmed by Mycobacterium tuberculosis culture from clinical samples.RESULTS:
The sensitivity of the Myco G test was 71% in active TB (pulmonary and extra-pulmonary), while the specificity was 100%. The Patho test showed a sensitivity of 43.5% with a specificity of 96.3%. A combination of both tests showed a sensitivity of 81% and a specificity of 96.3%.CONCLUSIONS:
Both ELISA tests were simple and easy to perform. Their combined use led to an increase in the diagnostic accuracy of active TB and its discrimination from non-TB pulmonary diseases. They could therefore be used as screening tools in poorly equipped laboratories in TB-endemic regions.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tuberculosis
/
Inmunoglobulina G
/
Ensayo de Inmunoadsorción Enzimática
/
Antígenos Bacterianos
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
Límite:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
País/Región como asunto:
Africa
Idioma:
En
Revista:
Int J Tuberc Lung Dis
Año:
2010
Tipo del documento:
Article
País de afiliación:
Túnez