T-vector and in vivo recombination as tools to construct a large antibody library of breast cancer.
Hybridoma (Larchmt)
; 29(3): 251-4, 2010 Jun.
Article
en En
| MEDLINE
| ID: mdl-20569001
ABSTRACT
The emergence of phage antibody libraries is an important advance in the field of antibody engineering. It provides a useful methodology to produce human antibodies and has the potential to replace traditional hybridoma technology. In our research, we used T-vector and in vivo recombination to construct a large antibody library from breast cancer patients. The use of T-vector considerably increased the cloning efficiency, and the diversity of the library could be increased easily using in vivo recombination. Taken together, a combination of these two techniques might be valuable in constructing a large antibody library.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Recombinación Genética
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Neoplasias de la Mama
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Región Variable de Inmunoglobulina
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Biblioteca de Péptidos
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Anticuerpos Monoclonales
Límite:
Adult
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Aged
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Female
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Humans
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Middle aged
Idioma:
En
Revista:
Hybridoma (Larchmt)
Año:
2010
Tipo del documento:
Article
País de afiliación:
China