Characterization of monoclonal antibodies specific for the Merkel cell polyomavirus capsid.
Virology
; 405(1): 20-5, 2010 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-20598728
ABSTRACT
Merkel cell polyomavirus (MCV) has been implicated as a causative agent in Merkel cell carcinoma. Robust polyclonal antibody responses against MCV have been documented in human subjects, but monoclonal antibodies (mAbs) specific for the VP1 capsid protein have not yet been characterized. We generated 12 mAbs capable of binding recombinant MCV virus-like particles. The use of a short immunogenic priming schedule was important for production of the mAbs. Ten of the 12 mAbs were highly effective for immunofluorescent staining of cells expressing capsid proteins. An overlapping set of 10 mAbs were able to neutralize the infectivity of MCV-based reporter vectors, with 50% effective doses in the low picomolar range. Three mAbs interfered with the binding of MCV virus-like particles to cells. This panel of anti-capsid antibodies should provide a useful set of tools for the study of MCV.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Poliomavirus
/
Proteínas de la Cápside
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Anticuerpos Monoclonales
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Anticuerpos Antivirales
Límite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Virology
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos