Expression of cyclooxygenase isoforms in the baculovirus expression system.
Methods Mol Biol
; 644: 45-54, 2010.
Article
en En
| MEDLINE
| ID: mdl-20645164
ABSTRACT
Prostaglandin synthase-1 and 2, also known as cyclooxygenase-1 and 2 (COX) catalyze the rate limiting step in the conversion of arachidonic acid to prostaglandins and other potent lipid mediators. As such, COX enzymes represent important pharmacological targets in the treatment of pain, inflammation, and fever. However, due to the typically low expression level of COX enzymes in most primary tissues, large amounts of highly purified enzyme for in depth study is difficult to obtain. Overexpression of COX in the baculovirus expression system represents a quick and efficient way to overcome this problem and obtain large amounts of catalytically active enzyme. Here, we present a step-by-step approach for COX expression in the baculovirus system.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Expresión Génica
/
Baculoviridae
/
Clonación Molecular
/
Prostaglandina-Endoperóxido Sintasas
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos