Suppression of SOS-inducing activity of chemical mutagens by metabolites from microbial transformation of (+)-longicyclene.
J Agric Food Chem
; 58(16): 9001-5, 2010 Aug 25.
Article
en En
| MEDLINE
| ID: mdl-20662538
ABSTRACT
In this study, biotransformation of (+)-longicyclene (1) by Aspergillus niger (NBRC 4414) and the suppressive effect on umuC gene expression by chemical mutagens 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide) and aflatoxin B1 (AFB1) of the SOS response in Salmonella typhimurium TA1535/pSK1002 were investigated. Initially, compound 1 was converted to three new terpenoids, (-)-(10R)-10-hydroxy-longicyclic acid (2), (+)-(10S)-10-hydroxy-longicyclic acid (3), and (+)-10-oxo-longicyclic acid (4) by A. niger , and their conversion rates were 27, 23, and 30%, respectively. The metabolites suppressed the SOS-inducing activity of furylfuramide and AFB1 in the umu test. Compounds 1-4 were hardly showing a suppressive effect on umu gene expression of the SOS responses in S. typhimurium TA1535/pSK1002 against furylfuramid. However, metabolites showed a suppressive effect against AFB1. Compound 4 had gene expression by chemical mutagen AFB1, was suppressed 53% at <1.0 mM, and was the most effective compound in this experiment.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sesquiterpenos
/
Respuesta SOS en Genética
/
Antimutagênicos
/
Mutágenos
Idioma:
En
Revista:
J Agric Food Chem
Año:
2010
Tipo del documento:
Article
País de afiliación:
Japón