In situ evaluation of chromium-DNA damage using a DNA-electrochemical biosensor.
Anal Bioanal Chem
; 398(4): 1633-41, 2010 Oct.
Article
en En
| MEDLINE
| ID: mdl-20686755
ABSTRACT
The in situ evaluation of the direct interaction of chromium species with double-stranded DNA (dsDNA) was studied using differential pulse voltammetry at a glassy carbon electrode. The DNA damage was electrochemically detected following the changes in the oxidation peaks of guanosine and adenosine bases. The results obtained revealed the interaction with dsDNA of the Cr(IV) and Cr(V) reactive intermediates of Cr(III) oxidation by O(2) dissolved in the solution bound to dsDNA. This interaction leads to different modifications and causes oxidative damage in the B-DNA structure. Using polyhomonucleotides of guanine and adenine, it was shown that the interaction between reactive intermediates Cr(IV) and Cr(V)-DNA causes oxidative damage and preferentially takes place at guanine-rich segments, leading to the formation of 8-oxoguanine, the oxidation product of guanine residues and a biomarker of DNA oxidative damage. The interaction of Cr(VI) with dsDNA causes breaking of hydrogen bonds, conformational changes, and unfolding of the double helix, which enables easier access of other oxidative agents to interact with DNA, and the occurrence of oxidative damage to DNA.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Daño del ADN
/
ADN
/
Técnicas Biosensibles
/
Cromo
/
Técnicas Electroquímicas
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2010
Tipo del documento:
Article
País de afiliación:
Portugal