Misregulation of the LOB domain gene DDA1 suggests possible functions in auxin signalling and photomorphogenesis.
J Exp Bot
; 62(1): 221-33, 2011 Jan.
Article
en En
| MEDLINE
| ID: mdl-20797997
ABSTRACT
The LATERAL ORGAN BOUNDARIES DOMAIN (LBD) gene family encodes plant-specific transcription factors. In this report, the LBD gene DOWN IN DARK AND AUXIN1 (DDA1), which is closely related to LATERAL ORGAN BOUNDARIES (LOB) and ASYMMETRIC LEAVES2 (AS2), was characterized. DDA1 is expressed primarily in vascular tissues and its transcript levels were reduced by exposure to exogenous indole-3-acetic acid (IAA or auxin) and in response to dark exposure. Analysis of a T-DNA insertion line, dda1-1, in which the insertion resulted in misregulation of DDA1 transcripts in the presence of IAA and in the dark revealed possible functions in auxin response and photomorphogenesis. dda1-1 plants exhibited reduced sensitivity to auxin, produced fewer lateral roots, and displayed aberrant hypocotyl elongation in the dark. Phenotypes resulting from fusion of a transcriptional repression domain to DDA1 suggest that DDA1 may act as both a transcriptional activator and a transcriptional repressor depending on the context. These results indicate that DDA1 may function in both the auxin signalling and photomorphogenesis pathways.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
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Transducción de Señal
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Arabidopsis
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Regulación de la Expresión Génica de las Plantas
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Proteínas de Arabidopsis
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Ácidos Indolacéticos
Idioma:
En
Revista:
J Exp Bot
Asunto de la revista:
BOTANICA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos