Characterization of metal-humic acid complexes by polyacrylamide gel electrophoresis-laser ablation-inductively coupled plasma mass spectrometry.
Anal Chim Acta
; 676(1-2): 9-14, 2010 Aug 31.
Article
en En
| MEDLINE
| ID: mdl-20800736
ABSTRACT
A new speciation methodology based on the use of 1D-PAGE (one dimensional-polyacrylamide gel electrophoresis), IEF (isoelectric focusing electrophoresis) and 2D-PAGE (two dimensional-polyacrylamide gel electrophoresis) as separation techniques and LA-ICP-MS (laser ablation-inductively coupled plasma mass spectrometry) as detection system have been applied to study the distribution of metal-humic acids (metal-HA) complexes in environmental samples. The use of 1D-Tris borate method using low current (10 mA) and Tris borate as trailing ion previous to LA-ICP-MS measurements, allowed to obtain the distribution of metals-HA complexes from a compost sample maintaining metals-HA complexes stability. It was observed that metals were associated with the smallest HA size fraction (around 3 kDa), validating results obtained by high performance size-exclusion chromatography (HP-SEC), cross-flow field flow fractionation (FIFFF) and ultrafiltration. IEF-LA-ICP-MS profiles indicated that there are two main regions (A) with 3
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Anal Chim Acta
Año:
2010
Tipo del documento:
Article
País de afiliación:
España