Detection of metallo-ß-lactamase genes in clinical specimens by a commercial multiplex PCR system.
J Microbiol Methods
; 83(2): 185-7, 2010 Nov.
Article
en En
| MEDLINE
| ID: mdl-20807554
ABSTRACT
hyplex®-MBL ID Multiplex PCR-ELISA, a novel method for identifying metallo-ß-lactamase genes directly in clinical specimens, was evaluated using a consecutive collection of 326 samples from three hospitals in Greece characterized by high prevalence of VIM producers. The method exhibited high sensitivity (98.0%) and specificity (98.6%) and was proven reliable in detecting bla(VIM) genes in blood, urine, pus, and sputum samples that, as confirmed by conventional methods, contained various VIM-producing species. Future multicenter studies should be considered for the thorough evaluation of this method and its potential diagnostic utility.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Beta-Lactamasas
/
Reacción en Cadena de la Polimerasa
/
Infecciones por Bacterias Gramnegativas
/
Bacterias Gramnegativas
Tipo de estudio:
Clinical_trials
/
Diagnostic_studies
/
Evaluation_studies
/
Risk_factors_studies
Límite:
Humans
País/Región como asunto:
Europa
Idioma:
En
Revista:
J Microbiol Methods
Año:
2010
Tipo del documento:
Article
País de afiliación:
Grecia