Selective peracetic acid determination in the presence of hydrogen peroxide using a label free enzymatic method based on catalase.
Anal Bioanal Chem
; 398(5): 2117-24, 2010 Nov.
Article
en En
| MEDLINE
| ID: mdl-20824427
ABSTRACT
Peracetic acid (PAA) is selectively determined in the presence of hydrogen peroxide (H(2)O(2)) by using the self-indicating UV-Vis molecular absorption properties of catalase. The PAA reacts with the protein giving an intermediate (Cat-I) which is reduced back by the amino acid core surrounding the heme group. Since the original form of the enzyme and the Cat-I have different UV-Vis absorption properties, the absorbance changes can be used for PAA determination. The H(2)O(2)/catalase reaction is extremely fast so that neither Cat-I compound nor kinetic interferences are observed. The method permits PAA determination in the 5 × 10(-7) to 1.5 × 10(-5) M range, the reproducibility being between 1% and 10%. Using this method, PAA has been successfully determined in water samples treated with commercial PAA/H(2)O(2) biocides. A theoretical study has also been carried out for obtaining a mathematical model able to analytically describe the process.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácido Peracético
/
Catalasa
/
Técnicas de Química Analítica
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Peróxido de Hidrógeno
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Modelos Teóricos
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2010
Tipo del documento:
Article
País de afiliación:
España