Your browser doesn't support javascript.
loading
Simultaneous optical spectroscopic measurement of hemoglobin and myoglobin saturations and cytochrome aa3 oxidation in vivo.
Arakaki, Lorilee S L; Ciesielski, Wayne A; Thackray, Brett D; Feigl, Eric O; Schenkman, Kenneth A.
Afiliación
  • Arakaki LS; Department of Pediatrics, University of Washington, Seattle, Washington 98195, USA. larakaki@u.washington.edu
Appl Spectrosc ; 64(9): 973-9, 2010 Sep.
Article en En | MEDLINE | ID: mdl-20828433
ABSTRACT
A method to simultaneously measure oxygenation in vascular, intracellular, and mitochondrial spaces from optical spectra acquired from muscle has been developed. In order to validate the method, optical spectra in the visible and near-infrared regions (600-850 nm) were acquired from solutions of myoglobin, hemoglobin, and cytochrome oxidase that included Intralipid as a light scatterer. Spectra were also acquired from the rabbit forelimb. Three partial least squares (PLS) analyses were performed on second-derivative spectra, each separately calibrated to myoglobin oxygen saturation, hemoglobin oxygen saturation, or cytochrome aa3 oxidation. The three variables were measured from in vitro and in vivo spectra that contained all three chromophores. In the in vitro studies, measured values of myoglobin saturation, hemoglobin saturation, and cytochrome aa3 oxidation had standard errors of 5.9%, 7.4%, and 12.2%, respectively, with little cross-talk between the in vitro measurements. In the progression from normal oxygenation to ischemia in the rabbit forelimb, hemoglobin desaturated first, followed by myoglobin, while cytochrome aa3 reduction occurred last. The ability to simultaneously measure oxygenations in the vascular, intracellular, and mitochondrial compartments will be valuable in physiological studies of muscle metabolism and in clinical studies when oxygen supply or utilization are compromised.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxígeno / Hemoglobinas / Complejo IV de Transporte de Electrones / Espectroscopía Infrarroja Corta / Mioglobina Límite: Animals Idioma: En Revista: Appl Spectrosc Año: 2010 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxígeno / Hemoglobinas / Complejo IV de Transporte de Electrones / Espectroscopía Infrarroja Corta / Mioglobina Límite: Animals Idioma: En Revista: Appl Spectrosc Año: 2010 Tipo del documento: Article País de afiliación: Estados Unidos