A direct role for cohesin in gene regulation and ecdysone response in Drosophila salivary glands.
Curr Biol
; 20(20): 1787-98, 2010 Oct 26.
Article
en En
| MEDLINE
| ID: mdl-20933422
ABSTRACT
BACKGROUND:
Developmental abnormalities observed in Cornelia de Lange syndrome have been genetically linked to mutations in the cohesin machinery. These and other recent experimental findings have led to the suggestion that cohesin, in addition to its canonical function of mediating sister chromatid cohesion, might also be involved in regulating gene expression.RESULTS:
We report that cleavage of cohesin's kleisin subunit in postmitotic Drosophila salivary glands induces major changes in the transcript levels of many genes. Kinetic analyses of changes in transcript levels upon cohesin cleavage reveal that a subset of genes responds to cohesin cleavage within a few hours. In addition, cohesin binds to most of these loci, suggesting that cohesin is directly regulating their expression. Among these genes are several that are regulated by the steroid hormone ecdysone. Cytological visualization of transcription at selected ecdysone-responsive genes reveals that puffing at Eip74EF ceases within an hour or two of cohesin cleavage, long before any decline in ecdysone receptor could be detected at this locus.CONCLUSION:
We conclude that cohesin regulates expression of a distinct set of genes, including those mediating the ecdysone response.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Glándulas Salivales
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Proteínas Cromosómicas no Histona
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Cromátides
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Regulación del Desarrollo de la Expresión Génica
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Proteínas de Ciclo Celular
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Proteínas de Drosophila
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Drosophila melanogaster
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Ecdisona
Límite:
Animals
Idioma:
En
Revista:
Curr Biol
Asunto de la revista:
BIOLOGIA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Reino Unido