Heme conjugated magnetic beads to isolate heme-binding proteins from complex mixtures.
Protein Expr Purif
; 76(1): 79-82, 2011 Mar.
Article
en En
| MEDLINE
| ID: mdl-20937390
ABSTRACT
The cofactor heme (Fe-protoporphyrin IX) plays many important roles in biology. Identification of novel proteins for the transport, chaperoning and delivery of heme in cells is of widespread interest. Here, we describe the use of heme conjugated magnetic beads for the isolation of heme-binding proteins from complex protein mixtures. The reagent is straightforward to use, sensitive and specific.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Portadoras
/
Proteínas Periplasmáticas
/
Hemo
Idioma:
En
Revista:
Protein Expr Purif
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2011
Tipo del documento:
Article
País de afiliación:
Reino Unido