Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
J Gen Appl Microbiol
; 56(4): 331-8, 2010 Aug.
Article
en En
| MEDLINE
| ID: mdl-20953097
ABSTRACT
Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene encoding ACC deaminase, but this gene is under the activity of the NifA-RpoN-dependent promoter; thus, it is only expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was integrated into the M. loti chromosome under a constitutive promoter activity. The resulting strain induced the formation of a higher number of nodules and was more competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results suggest that the introduction of the ACC deaminase activity within M. loti in a constitutive way could be a novel strategy to increase nodulation competitiveness of the bacteria, which could be useful for the forage inoculants industry.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Simbiosis
/
Liasas de Carbono-Carbono
/
Alphaproteobacteria
/
Lotus
Idioma:
En
Revista:
J Gen Appl Microbiol
Año:
2010
Tipo del documento:
Article
País de afiliación:
Argentina