Identification and characterisation of the BPI/LBP/PLUNC-like gene repertoire in chickens reveals the absence of a LBP gene.
Dev Comp Immunol
; 35(3): 285-95, 2011 Mar.
Article
en En
| MEDLINE
| ID: mdl-20959152
ABSTRACT
Palate, lung and nasal epithelial clone (PLUNC) proteins are structural homologues to the innate defence molecules LPS-binding protein (LBP) and bactericidal/permeability-increasing protein (BPI). PLUNCs make up the largest portion of the wider BPI/LBP/PLUNC-like protein family and are amongst the most rapidly evolving mammalian genes. In this study we systematically identified and characterised BPI/LBP/PLUNC-like protein-encoding genes in the chicken genome. We identified eleven complete genes (and a pseudogene). Five of them are clustered on a >50 kb locus on chromosome 20, immediately adjacent to BPI. In addition to BPI, we have identified presumptive orthologues LPLUNCs 2, 3, 4 and 6, and BPIL-2. We find no evidence for the existence of single domain containing proteins in birds. Strikingly our analysis also suggests that there is no LBP orthologue in chicken. This observation may in part account for the relative resistance to LPS toxicity observed in birds. Our results indicate significant differences between the avian and mammalian repertoires of BPI/LBP/PLUNC-like genes at the genomic and transcriptional levels and provide a framework for further functional analyses of this gene family in chickens.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Fase Aguda
/
Glicoproteínas de Membrana
/
Proteínas Sanguíneas
/
Proteínas Portadoras
/
Pollos
/
Péptidos Catiónicos Antimicrobianos
/
Proteínas Aviares
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Reino Unido