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V-fusion: a convenient, nontoxic method for cell fusion.
Gottesman, Amy; Milazzo, Joseph; Lazebnik, Yuri.
Afiliación
  • Gottesman A; Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11768, USA.
Biotechniques ; 49(4): 747-50, 2010 Oct.
Article en En | MEDLINE | ID: mdl-20964635
Cell-to-cell fusion (cell fusion) is a fundamental biological process that also has been used as a versatile experimental tool to dissect a variety of cellular mechanisms, including the consequences of cell fusion itself, and to produce cells with desired properties, such as hybridomas and reprogrammed progenitors. However, current methods of cell fusion are not satisfactory because of their toxicity, inefficiency, or lack of flexibility. We describe a simple, versatile, scalable, and nontoxic approach that we call V-fusion, as it is based on the ability of the vesicular stomatitis virus G protein (VSV-G), a viral fusogen of broad tropism, to become rapidly and reversibly activated. We suggest that this approach will benefit a broad array of studies that investigate consequences of cell fusion or use cell fusion as an experimental tool.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción Genética / Glicoproteínas de Membrana / Fusión Celular / Proteínas del Envoltorio Viral Límite: Humans Idioma: En Revista: Biotechniques Año: 2010 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción Genética / Glicoproteínas de Membrana / Fusión Celular / Proteínas del Envoltorio Viral Límite: Humans Idioma: En Revista: Biotechniques Año: 2010 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido