Mu opioid receptor mRNA expression, binding, and functional coupling to G-proteins in human epileptic hippocampus.
Hippocampus
; 22(2): 122-7, 2012 Feb.
Article
en En
| MEDLINE
| ID: mdl-21049484
ABSTRACT
Mu opioid receptors (MOR) are known to be involved in seizure activity. The main goal of the present study was to characterize the MOR mRNA expression, binding, as well as G protein activation mediated by these receptors in epileptic hippocampus of patients with pharmacoresistant mesial temporal lobe epilepsy (TLE). In contrast with autopsy samples, hippocampus obtained from patients with mesial TLE demonstrated enhanced MOR mRNA expression (116%). Saturation binding experiments revealed significantly higher (60%) B(max) values for the mesial TLE group, whereas the K(d) values were not statistically different. Although mesial TLE group demonstrated high levels of basal binding for the G proteins (136%), DAMGO-stimulated [(35)S]GTPγS binding did not demonstrate significant alterations. In conclusion, our present data provide strong evidence that the epileptic hippocampus of patients with pharmacoresistant mesial TLE presents significant alterations in MOR. Such changes may represent adaptive mechanisms to compensate for other as yet unknown alterations.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN Mensajero
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Receptores Opioides mu
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Proteínas de Unión al GTP
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Epilepsia del Lóbulo Temporal
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Hipocampo
Límite:
Adolescent
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Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Hippocampus
Asunto de la revista:
CEREBRO
Año:
2012
Tipo del documento:
Article
País de afiliación:
México