Vitamin D3 treatment of Crohn's disease patients increases stimulated T cell IL-6 production and proliferation.
Aliment Pharmacol Ther
; 32(11-12): 1364-72, 2010 Dec.
Article
en En
| MEDLINE
| ID: mdl-21050239
ABSTRACT
BACKGROUND:
Vitamin D3 has shown immune-modulating effects in CD4+ T cells from Crohn's disease patients in vitro.AIM:
To investigate the effects of in vivo vitamin D3 treatment on T cells in Crohn's disease patients.METHODS:
Peripheral blood mononuclear cells (PBMC) were isolated at week 0 and at week 26 from 10 vitamin D3- and 10 placebo-treated Crohn's disease patients participating in a randomized, placebo-controlled, clinical trial study. Monocyte-depleted PBMC were stimulated with anti-CD3 and anti-CD28, and cultured for 7, days, to investigate CD4+ T-cell proliferation and T-cell cytokine production.RESULTS:
In vitamin D3-treated patients, the median 25-hydroxyvitamin D3 levels increased 70 nmol/L compared with -5 nmol/L in the placebo group. Vitamin D3 treatment increased interleukin-6 production (delta = 188 pg/mL, range -444 to 4071) compared with a decrease in the placebo group (delta = -896 pg/mL, range -3841 to 1323) (P < 0.02, Wilcoxon rank sum test). Interestingly, vitamin D3 increased the amount of proliferating stimulated CD4+ T cells from median 41% (range 10-75%) to 56% (range 26-77%) (P = 0.02, Wilcoxon rank sum test).CONCLUSIONS:
Vitamin D3 treatment of Crohn's disease patients increased the IL-6 levels. Interestingly, vitamin D3 treatment enhanced the CD4+ T cell proliferation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Linfocitos T
/
Enfermedad de Crohn
/
Interleucina-6
/
Colecalciferol
Tipo de estudio:
Clinical_trials
Límite:
Adult
/
Aged
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Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Aliment Pharmacol Ther
Asunto de la revista:
FARMACOLOGIA
/
GASTROENTEROLOGIA
/
TERAPIA POR MEDICAMENTOS
Año:
2010
Tipo del documento:
Article
País de afiliación:
Dinamarca
Pais de publicación:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM