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Brightness-compensated 3-D optical flow algorithm for monitoring cochlear motion patterns.
von Tiedemann, Miriam; Fridberger, Anders; Ulfendahl, Mats; de Monvel, Jacques Boutet.
Afiliación
  • von Tiedemann M; Karolinska Institutet, Center for Hearing and Communication Research, Department of Clinical Neuroscience and Department of Otolaryngology, SE-171 76 Stockholm, Sweden.
J Biomed Opt ; 15(5): 056012, 2010.
Article en En | MEDLINE | ID: mdl-21054106
A method for three-dimensional motion analysis designed for live cell imaging by fluorescence confocal microscopy is described. The approach is based on optical flow computation and takes into account brightness variations in the image scene that are not due to motion, such as photobleaching or fluorescence variations that may reflect changes in cellular physiology. The 3-D optical flow algorithm allowed almost perfect motion estimation on noise-free artificial sequences, and performed with a relative error of <10% on noisy images typical of real experiments. The method was applied to a series of 3-D confocal image stacks from an in vitro preparation of the guinea pig cochlea. The complex motions caused by slow pressure changes in the cochlear compartments were quantified. At the surface of the hearing organ, the largest motion component was the transverse one (normal to the surface), but significant radial and longitudinal displacements were also present. The outer hair cell displayed larger radial motion at their basolateral membrane than at their apical surface. These movements reflect mechanical interactions between different cellular structures, which may be important for communicating sound-evoked vibrations to the sensory cells. A better understanding of these interactions is important for testing realistic models of cochlear mechanics.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cóclea / Microscopía Confocal Tipo de estudio: Evaluation_studies / Prognostic_studies Límite: Animals Idioma: En Revista: J Biomed Opt Asunto de la revista: ENGENHARIA BIOMEDICA / OFTALMOLOGIA Año: 2010 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cóclea / Microscopía Confocal Tipo de estudio: Evaluation_studies / Prognostic_studies Límite: Animals Idioma: En Revista: J Biomed Opt Asunto de la revista: ENGENHARIA BIOMEDICA / OFTALMOLOGIA Año: 2010 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos