Relationship between suppression of E6 and E7 virus oncogenes and expression of apoptosis and cell cycle genes in cervical cancer culture.
Bull Exp Biol Med
; 149(1): 73-7, 2010 Jul.
Article
en En, Ru
| MEDLINE
| ID: mdl-21113463
ABSTRACT
The effects of short interfering RNA suppressing the expression of E6 and E7 human papilloma virus (type 18) on the expression of apoptosis and cell cycle genes were studied in HeLa cells. Changes in the transcription profiles were evaluated using DNA microarray and real-time reverse-transcription PCR. Cell transfection with anti-E6 and anti-E7 short interfering RNA moderately reduced the expression of mRNA for CDC25C, GRB2, GTSE1, and PLK1 genes and induced expression of CDKN1A (p21(CIP)) gene mRNA. In addition, culture proliferation was inhibited and morphological changes characteristic of differentiation and cell aging developed.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Regulación Viral de la Expresión Génica
/
Proteínas Oncogénicas Virales
/
Apoptosis
/
Genes cdc
/
ARN Interferente Pequeño
/
Proteínas de Unión al ADN
Límite:
Humans
Idioma:
En
/
Ru
Revista:
Bull Exp Biol Med
Año:
2010
Tipo del documento:
Article
País de afiliación:
Rusia