High-resolution imaging of kidney vascular corrosion casts with Nano-CT.
Microsc Microanal
; 17(2): 215-9, 2011 Apr.
Article
en En
| MEDLINE
| ID: mdl-21122193
ABSTRACT
A vascular corrosion cast of an entire mouse kidney was scanned with a modular multiresolution X-ray nanotomography system. Using an isotropic voxel pitch of 0.5 µm, capillary systems such as the vasa recta, peritubular capillaries and glomeruli were clearly resolved. This represents a considerable improvement over corrosion casts scanned with microcomputed tomography systems. The resolving power of this system was clearly demonstrated by the unique observation of a dense, subcapsular mat of capillaries enveloping the entire outer surface of the cortical region. Resolution of glomerular capillaries was comparable to similar models derived from laser scanning confocal microscopy. The high-resolution, large field of view and the three-dimensional nature of the resulting data opens new possibilities for the use of corrosion casting in research.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vasos Sanguíneos
/
Riñón
Límite:
Animals
Idioma:
En
Revista:
Microsc Microanal
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos