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3D cryo-EM structure of an active step I spliceosome and localization of its catalytic core.
Golas, Monika M; Sander, Bjoern; Bessonov, Sergey; Grote, Michael; Wolf, Elmar; Kastner, Berthold; Stark, Holger; Lührmann, Reinhard.
Afiliación
  • Golas MM; Research Group of 3D Electron Cryomicroscopy, Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany.
Mol Cell ; 40(6): 927-38, 2010 Dec 22.
Article en En | MEDLINE | ID: mdl-21172658
The spliceosome excises introns from pre-mRNA in a two-step splicing reaction. So far, the three-dimensional (3D) structure of a spliceosome with preserved catalytic activity has remained elusive. Here, we determined the 3D structure of the human, catalytically active step I spliceosome (C complex) by cryo-electron microscopy (cryo-EM) in vitrified ice. Via immunolabeling we mapped the position of the 5' exon. The C complex contains an unusually salt-stable ribonucleoprotein (RNP) core that harbors its catalytic center. We determined the 3D structure of this RNP core and also that of a post-step II particle, the 35S U5 snRNP, which contains most of the C complex core proteins. As C complex domains could be recognized in these structures, their position in the C complex could be determined, thereby allowing the region harboring the spliceosome's catalytic core to be localized.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Empalmosomas / Biocatálisis Límite: Humans Idioma: En Revista: Mol Cell Asunto de la revista: BIOLOGIA MOLECULAR Año: 2010 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Empalmosomas / Biocatálisis Límite: Humans Idioma: En Revista: Mol Cell Asunto de la revista: BIOLOGIA MOLECULAR Año: 2010 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos