Proton inactivation of melanoma cells enhanced by fotemustine.
Radiat Prot Dosimetry
; 143(2-4): 503-7, 2011 Feb.
Article
en En
| MEDLINE
| ID: mdl-21183545
ABSTRACT
Response of human HTB140 melanoma cells to proton irradiation in combination with fotemustine (FM) was investigated. Effects of these agents were analysed on cell proliferation and induction of apoptosis. Cells pretreated with 100- or 250-µM of FM were irradiated in the middle of the therapeutic 62-MeV proton spread-out Bragg peak, with a dose of 16 Gy. All treatments reduced proliferation and survival of melanoma cells. The most pronounced effects of the combined treatment were obtained for cell survivals. The level of apoptosis increased after all applied treatments. Particularly good pro-apoptotic effect was achieved when proton irradiation was combined with 250 µM of FM. This was followed by the increased expression of p53 gene. The obtained results have shown that combined application of FM and protons significantly reduced growth of this resistant melanoma cell line.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos Organofosforados
/
Supervivencia Celular
/
Melanoma
/
Antineoplásicos
/
Compuestos de Nitrosourea
Límite:
Humans
Idioma:
En
Revista:
Radiat Prot Dosimetry
Año:
2011
Tipo del documento:
Article