[Determination of geniposide and croein-1 in different breed of fructus gardeniae].

OBJECTIVE: To determinate geniposide and croein-1 in Fructus Gardeniae from different breeds. METHODS: RP-HPLC method was adopted. The chromatographic separation was performed on an Eclipse XDB-C18 column (250 mm x 4.6 mm, 5 microm, Agilent). The mobile phase was a mixture of acetonitrile-0.1% phosphoric acid aqueous solution in gradient elution. The wavelength of 238 nm and 440 nm was selected to determinate geniposide and crocins simultaneously. RESULT: The contents of geniposide and croein-1 in different breeds of Fructus Gardeniae are different. CONCLUSION: This method is simple and repeatable, and could be used for the quality control of Fructus Gardeniae.