Photoaffinity labeling of the lysosomal neuraminidase from bovine testis.
FEBS Lett
; 277(1-2): 42-4, 1990 Dec 17.
Article
en En
| MEDLINE
| ID: mdl-2125279
ABSTRACT
ASA-NeuAc2en, a photoreactive arylazide derivative of sialic acid, is shown to be a powerful competitive inhibitor of lysosomal neuraminidase from bovine testis (Ki approximately 21 microM). Photoaffinity labeling and partial purification of preparations containing this lysosomal neuraminidase activity result in specifically and non-specifically labeled polypeptides. Only labeling in a 55 kDa polypeptide is found to be specific, since it could be prevented by the competitive neuraminidase inhibitor NeuAc2en. We conclude that the 55 kDa polypeptide in the bovine testis beta-galactosidase/neuraminidase/protective protein complex contains the catalytic site of neuraminidase.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Testículo
/
Neuraminidasa
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1990
Tipo del documento:
Article
País de afiliación:
Países Bajos