Four-way ligation for construction of a mammalian cell-based full-length antibody display library.
Acta Biochim Biophys Sin (Shanghai)
; 43(3): 232-8, 2011 Mar.
Article
en En
| MEDLINE
| ID: mdl-21257624
ABSTRACT
A unique four-way ligation strategy was developed for rapid construction of a full-length antibody library. A mammalian expression vector was constructed that contained dual mammalian expression cassettes and sequences recognized by the unique restriction enzymes BsmBI, BstXI, and SfiI. Both full-length light-chain and variable domain of heavy-chain genes were inserted into the vector in one step by four-way ligation, and full-length bivalent antibodies were displayed on mammalian cell surfaces. Using this strategy, only 2 weeks were required to successfully construct high-quality, full-length human antibody libraries.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transfección
/
Biblioteca de Genes
/
Vectores Genéticos
/
Anticuerpos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Acta Biochim Biophys Sin (Shanghai)
Asunto de la revista:
BIOFISICA
/
BIOQUIMICA
Año:
2011
Tipo del documento:
Article
País de afiliación:
China