Affinity of Cu+ for the copper-binding domain of the amyloid-ß peptide of Alzheimer's disease.
Inorg Chem
; 50(5): 1614-8, 2011 Mar 07.
Article
en En
| MEDLINE
| ID: mdl-21280585
ABSTRACT
The role of metal ions in Alzheimer's disease etiology is unresolved. For the redox-active metal ions iron and copper, the formation of reactive oxygen species by metal amyloid complexes has been proposed to contribute to Alzheimer's disease neurodegeneration. For copper, reactive oxygen species are generated by copper redox cycling between its 1+ and 2+ oxidation states. Thus, the AßCu(I) complex is potentially a critical reactant associated with Alzheimer's disease etiology. Through competitive chelation, we have measured the affinity of the soluble copper-binding domain of the amyloid-ß peptide for Cu(I). The dissociation constants are in the femtomolar range for both wild-type and histidine-to-alanine mutants. These results indicate that Cu(I) binds more tightly to monomeric amyloid-ß than Cu(II) does, which leads us to propose that Cu(I) is a relevant in vivo oxidation state.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptidos beta-Amiloides
/
Cobre
/
Enfermedad de Alzheimer
Idioma:
En
Revista:
Inorg Chem
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos