Ontogenetic changes in the scaling of cellular respiration with respect to size among sunflower seedlings.

ABSTRACT

The respiration rates R (oxygen uptake per min) and body mass M (mg per individual) of sunflower (Helianthus annuus L.) seedlings were measured for populations raised in the dark (scotomorphogenesis) and for plants subsequently grown in white light (photomorphogenesis) to determine the allometric (scaling) relationship for R vs. M. Based on ordinary least squares and reduced major axis regression protocols, cellular respiration rates were found to increase non-linearly as a 'broken-stick' curve of increasing M. During germination, the scaling was ca. 7.5-fold higher than after the emergence of the cotyledons from the seed coat, which can be attributed to the hypoxic conditions of the enclosed embryo. During seedling development, R was found to scale roughly as the 3/7 power of body mass (i.e., R ~ M(-3/7)), regardless of whether plants were grown in the dark or subsequently in white light. The numerical value of 3/7 statistically significantly differs from that reported across field- or laboratory-grown plants (i.e., R ~ M(-1.0)). It also differs from the expectations of recent allometric theory (i.e., R ~ M(-0.75) to M(-1.0)). This difference is interpreted to be the result of species-specific tissue-compositions that affect the volume fractions of metabolically active and less active cells. These findings, which are supported by cytological and ultrastructural observations (i.e., scanning- and transmission electron micrographs), draw attention to the need to measure R of developing plants in a tissue- or organ-specific context.