[Identification of pathogens causing root rot of Sophora tonkinensis].

ABSTRACT

OBJECTIVE: To identify the pathogens what caused of root rot, it can provide method of theoretical gist of integrated pest management of these kinds of diseases in the future. METHODS: Pathogens from rotten root of Sophora tonkinensis were isolated by tissue isolation. Their morphological characteristics were observed and rDNA-ITS sequence were sequenced, then analyzed by Blast in GenBank. RESULTS: Round colony in PDA medium. The aerial mycelium was thin, white, light gray and yellowish brown eustroma was on the surface of material. The surface of base material was flesh. Large number of small conidia ware oval, kidney-shaped, 8-16 microm x 2.5 -4 microm. And the large conidia just like Matt spore type, which had 3 to 5 septums. The length of rDNA-ITS of the fungi was 553 bp, which the ITS region sequences compared with the sequence of Fusarium solani (accession number AB518683.1, AB470903.1, AB369488.1, AJ608989.1, GQ365154.1, EF152426.1), and Fusarium oxysporum (accession number GQ922558.1, GQ922559.1, DQ452447.1) homology reached 99%. CONCLUSION: Combination of two identification methods,it arrived at the cause of root rot pathogen fungi was Fusarium solani.