Atorvastatin reduces high glucose toxicity in rat peritoneal mesothelial cells.
Perit Dial Int
; 31(3): 325-31, 2011.
Article
en En
| MEDLINE
| ID: mdl-21357937
ABSTRACT
OBJECTIVE:
Continuous exposure of the peritoneal membrane to high glucose dialysis solutions can produce functional alterations in this membrane. We studied the toxic effects of high glucose (50 mmol/L and 83 mmol/L) and its reversal by atorvastatin (0.5 - 5 µmol/L) on cultures of rat peritoneal mesothelial cells (PMCs).METHODS:
Rat PMCs were harvested from the peritonea of male Sprague-Dawley rats and grown in M199 medium supplemented with 10% fetal bovine serum. The effects of high glucose (50 mmol/L and 83 mmol/L) on levels of reactive oxygen species (ROS), on caspase 3 activity, and on phospho-p38 mitogen-activated protein kinase (MAPK) in the cultures were evaluated.RESULTS:
Exposure to high glucose (for 4, 8, and 24 hours) increased intracellular levels of ROS and phospho-p38 MAPK (indices of cellular toxicity). Atorvastatin blocked these toxic effects of high glucose, being more effective against 50 mmol/L glucose (protective effects were observed above 0.5 µmol/L) than against 83 mmol/L (protective effects were observed above 2.5 µmol/L). Atorvastatin was also able to prevent glucose-induced increase in caspase 3 activity.CONCLUSIONS:
The present study shows that high glucose may promote oxidative stress and may activate apoptotic pathways in rat PMCs. These toxic effects could be reversed by atorvastatin.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Peritoneo
/
Pirroles
/
Inhibidores de Hidroximetilglutaril-CoA Reductasas
/
Células Epiteliales
/
Glucosa
/
Ácidos Heptanoicos
Límite:
Animals
Idioma:
En
Revista:
Perit Dial Int
Asunto de la revista:
NEFROLOGIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
España