In vivo effect of 2-deoxy-D-glucose on glucose-6-phosphate dehydrogenase activity in the cytosol of liver, heart and skeletal muscle of rats.
Acta Physiol Pol
; 41(7): 138-45, 1990.
Article
en En
| MEDLINE
| ID: mdl-2136308
ABSTRACT
2-deoxy-D-glucose (2-DG), the unmetabolizable analogue of glucose induces a series of metabolic, hormonal and behavioral responses, causing cellular glucoprivation. According to in vitro studies, 2-DG inhibits phosphofructokinase in cultured human cells. The present investigations deal with changes in the cytosolic glucose-6-phosphate dehydrogenase activity following in vivo 2-DG administration. A single dose of 2-DG (600 mg/kg) has no influence on the activity of glucose-6-phosphate dehydrogenase in the cytosol of liver, heart and skeletal muscle of the rat. The concomitant increase in serum glucose, lactate and FFA concentrations observed in the study indicates indirectly a stimulation of adrenergic system. After three days of successive administration of 2-DG to rats, dehydrogenase activity decreased in the liver by approx 57% and in the skeletal muscle by approx 82% in comparison with control animals. Moreover the in vivo effect of 2-DG was found to be fully reversible, probably when the total amount of the inhibitor was excreted.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Citosol
/
Desoxiglucosa
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Glucosafosfato Deshidrogenasa
/
Hígado
/
Músculos
/
Miocardio
Límite:
Animals
Idioma:
En
Revista:
Acta Physiol Pol
Año:
1990
Tipo del documento:
Article
Pais de publicación:
PL
/
POLAND
/
POLONIA
/
POLÔNIA