Proofreading deficiency of Pol I increases the levels of spontaneous rpoB mutations in E. coli.
Mutat Res
; 712(1-2): 28-32, 2011 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-21459099
ABSTRACT
The fidelity role of DNA polymerase I in chromosomal DNA replication in E. coli was investigated using the rpoB forward target. These experiments indicated that in a strain carrying a proofreading-exonuclease-defective form of Pol I (polAexo mutant) the frequency of rpoB mutations increased by about 2-fold, consistent with a model that the fidelity of DNA polymerase I is important in controlling the overall fidelity of chromosomal DNA replication. DNA sequencing of rpoB mutants revealed that the Pol I exonuclease deficiency lead to an increase in a variety of base-substitution mutations. A polAexo mutator effect was also observed in strains defective in DNA mismatch repair and carrying the dnaE915 antimutator allele. Overall, the data are consistent with a proposed role of Pol I in the faithful completion of Okazaki fragment gaps at the replication fork.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Escherichia coli
/
ADN Polimerasa I
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Replicación del ADN
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Escherichia coli
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Mutación
Idioma:
En
Revista:
Mutat Res
Año:
2011
Tipo del documento:
Article
País de afiliación:
Polonia