Modular electron transfer circuits for synthetic biology: insulation of an engineered biohydrogen pathway.
Bioeng Bugs
; 1(6): 413-8, 2010.
Article
en En
| MEDLINE
| ID: mdl-21468209
ABSTRACT
Electron transfer is central to a wide range of essential metabolic pathways, from photosynthesis to fermentation. The evolutionary diversity and conservation of proteins that transfer electrons makes these pathways a valuable platform for engineered metabolic circuits in synthetic biology. Rational engineering of electron transfer pathways containing hydrogenases has the potential to lead to industrial scale production of hydrogen as an alternative source of clean fuel and experimental assays for understanding the complex interactions of multiple electron transfer proteins in vivo. We designed and implemented a synthetic hydrogen metabolism circuit in Escherichia coli that creates an electron transfer pathway both orthogonal to and integrated within existing metabolism. The design of such modular electron transfer circuits allows for facile characterization of in vivo system parameters with applications toward further engineering for alternative energy production.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ingeniería Genética
/
Transporte de Electrón
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Escherichia coli
/
Biología Sintética
/
Hidrógeno
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
Bioeng Bugs
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos