Targeted imaging of colorectal dysplasia in living mice with fluorescence microendoscopy.
Biomed Opt Express
; 2(4): 981-6, 2011 Mar 28.
Article
en En
| MEDLINE
| ID: mdl-21483619
ABSTRACT
We validate specific binding activity of a fluorescence-labeled peptide to colorectal dysplasia in living mice using a miniature, flexible, fiber microendoscope that passes through the instrument channel of an endoscope. The microendoscope delivers excitation light at 473 nm through a fiber-optic bundle with outer diameter of 680 µm to collect en face images at 10 Hz with 4 µm lateral resolution. We applied the FITC-labeled peptide QPIHPNNM topically to colonic mucosa in genetically engineered mice that spontaneously develop adenomas. More than two-fold greater fluorescence intensity was measured from adenomas compared to adjacent normal-appearing mucosa. Images of adenomas showed irregular morphology characteristic of dysplasia.
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Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Biomed Opt Express
Año:
2011
Tipo del documento:
Article