[Construstion of IL3-PE38KDEL fusion gene eukaryolic expression vector and its expression in pichia].

ABSTRACT

AIM: to construct a eukaryolic expression plasmid of Ppic9k-IL3-Linker-PE38KDE. METHODS: the IL3 and PE38KDEL gene were amplified by polymerase chain reaction (PCR) and cloned into the eukaryolic expression plasmid Ppic9k-Linker constructed after being sequenced.The recombinant vector confirmed by restriction endonucleases digestion, coenobium PCR and DNA sequence analysis showed that the prokaryotic expression vector Ppic9k-IL3-Linker-PE38KDEL was constructed successfully. CONCLUSION: the fusion gene IL3-PE38KDEL is successfully constructed, which laid a solid foundation for the further research.