Immuno-potentiating role of encapsulated proteins of infectious diseases in biopolymeric nanoparticles as a potential delivery system.

ABSTRACT

The study aimed to evaluate encapsulation of therapeutic proteins in nanoparticles to understand the interactions among Cytokines in the immune response to chronic infection, induction of long lasting memory cell formation and cytokine production. Chitosan nanoparticles in the size range of approximately 200 nm were prepared by ionic gelation method which binds with M. tuberculosis CFP-10 and CFP-21 proteins. Mean Residence Time were found to be higher for nanoparticles coated CFP-10 and CFP-21 proteins resulting in increase in relative bioavailability of encapsulated proteins in comparison with free proteins. Proteins encapsulated nanoparticles exerted negligible cytotoxicity as compared to 80% cytotoxicity by CFP-10 and CFP-21 proteins per se.