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Loss of galectin-3 expression in mucinous colorectal carcinomas is associated with 5'CpG island methylation in Tunisian patients.
Mahmoud, Lilia Kria Ben; Arfaoui, Amira; Khiari, Mariem; Chaar, Ines; El Amine, Olfa; Hmida, Abdel Majid Ben; Gharbi, Lasaad; Mzabi, Sabeh Regaya; Bouraoui, Saadia.
Afiliación
  • Mahmoud LK; Department of Colorectal Cancer Research, UR03ES04 Science University El Manar, Tunisia. liliakria77@yahoo.com
Appl Immunohistochem Mol Morphol ; 19(3): 258-65, 2011 May.
Article en En | MEDLINE | ID: mdl-21494181
The ß-galactoside-binding protein galectin-3 (gal-3) has pleitropic biological functions and has been implicated in cell growth, differentiation, adhesion, RNA processing, apoptosis, and malignant transformation. To investigate the pattern of inactivation of the gal-3 gene (LGALS3) in colorectal cancers (CRC), we studied a series of Tunisian patients with CRC to identify abnormal methylation in LGALS3 promoter using a methylation-specific PCR. We also examined the gal-3 gene expression by reverse transcription-PCR and the expression of gal-3 protein by immunohistochemistry. Analysis of DNA methylation in nonmucinous colorectal carcinomas expressing gal-3 protein showed an unmethylated profile of LGALS3 promoter, whereas gal-3 was aberrantly methylated in mucinous colorectal carcinomas. Complete loss of the gal-3 expression both at mRNA and the protein level was associated with the gal-3 methylation in the mucinous colorectal carcinomas. Our results show that methylation of the gal-3 promoter could be an important mechanism in the regulation of the expression of this gene in mucinous CRCs.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Regulación Neoplásica de la Expresión Génica / Colon / Adenocarcinoma Mucinoso / Galectina 3 Tipo de estudio: Risk_factors_studies País/Región como asunto: Africa Idioma: En Revista: Appl Immunohistochem Mol Morphol Asunto de la revista: BIOLOGIA MOLECULAR / HISTOCITOQUIMICA Año: 2011 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Regulación Neoplásica de la Expresión Génica / Colon / Adenocarcinoma Mucinoso / Galectina 3 Tipo de estudio: Risk_factors_studies País/Región como asunto: Africa Idioma: En Revista: Appl Immunohistochem Mol Morphol Asunto de la revista: BIOLOGIA MOLECULAR / HISTOCITOQUIMICA Año: 2011 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Estados Unidos