Loss of galectin-3 expression in mucinous colorectal carcinomas is associated with 5'CpG island methylation in Tunisian patients.
Appl Immunohistochem Mol Morphol
; 19(3): 258-65, 2011 May.
Article
en En
| MEDLINE
| ID: mdl-21494181
The ß-galactoside-binding protein galectin-3 (gal-3) has pleitropic biological functions and has been implicated in cell growth, differentiation, adhesion, RNA processing, apoptosis, and malignant transformation. To investigate the pattern of inactivation of the gal-3 gene (LGALS3) in colorectal cancers (CRC), we studied a series of Tunisian patients with CRC to identify abnormal methylation in LGALS3 promoter using a methylation-specific PCR. We also examined the gal-3 gene expression by reverse transcription-PCR and the expression of gal-3 protein by immunohistochemistry. Analysis of DNA methylation in nonmucinous colorectal carcinomas expressing gal-3 protein showed an unmethylated profile of LGALS3 promoter, whereas gal-3 was aberrantly methylated in mucinous colorectal carcinomas. Complete loss of the gal-3 expression both at mRNA and the protein level was associated with the gal-3 methylation in the mucinous colorectal carcinomas. Our results show that methylation of the gal-3 promoter could be an important mechanism in the regulation of the expression of this gene in mucinous CRCs.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Colorrectales
/
Regulación Neoplásica de la Expresión Génica
/
Colon
/
Adenocarcinoma Mucinoso
/
Galectina 3
Tipo de estudio:
Risk_factors_studies
País/Región como asunto:
Africa
Idioma:
En
Revista:
Appl Immunohistochem Mol Morphol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
HISTOCITOQUIMICA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Túnez
Pais de publicación:
Estados Unidos