Fluorescence in situ hybridisation in the cytological diagnosis of pancreatobiliary tumours.
Pathology
; 43(4): 335-9, 2011 Jun.
Article
en En
| MEDLINE
| ID: mdl-21519286
ABSTRACT
AIMS:
To assess the sensitivity, specificity, positive and negative predictive values of fluorescence in situ hybridisation (FISH) and conventional cytology in identifying bile duct stricture malignancies.METHODS:
Brushing samples were collected from 64 patients by means of endoscopic retrograde cholangiopancreatography, and assessed cytologically and by means of a multi-probe FISH set. The cytological diagnoses were positive, negative and suspicious, whereas criteria for FISH positivity were more than five polysomic cells or more than 10 trisomic cells for chromosomes 3 or 7.RESULTS:
Forty-eight of the 64 patients showed histological or clinical signs of malignancy. The sensitivity of cytology was high (77%) if suspicious cases were considered positive, but was significantly lower than that of FISH if suspicious cases were considered negative (58% versus 90%; p < 0.05). The specificity of cytology was 81% (positive and suspicious) or 100% (negative and suspicious), and the specificity of FISH was 94% (p = 1). FISH yielded one false negative result (isolated chromosome 7 trisomy). FISH allowed a definite diagnosis of 9/12 cytologically inconclusive cases.CONCLUSIONS:
Our findings suggest using FISH in the case of bile duct strictures cytologically negative or inconclusive; a FISH diagnosis of malignancy should only be made in the presence of polysomic pattern.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de los Conductos Biliares
/
Adenocarcinoma
/
Colangitis
/
Hibridación Fluorescente in Situ
/
Colangiocarcinoma
/
Pancreatitis Crónica
/
Liposarcoma
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Pathology
Año:
2011
Tipo del documento:
Article
País de afiliación:
Italia