Secretion of biologically-active human interferon-ß by Bacillus subtilis.
Biotechnol Lett
; 33(9): 1847-52, 2011 Sep.
Article
en En
| MEDLINE
| ID: mdl-21544609
ABSTRACT
Human interferon-ß (hIFN-ß) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-ß with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-ß with AmyE propeptide increased by more than 1.5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-ß in B. subtilis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacillus subtilis
/
Interferón beta
Límite:
Humans
Idioma:
En
Revista:
Biotechnol Lett
Año:
2011
Tipo del documento:
Article
País de afiliación:
Japón