Occupancy of chromatin organizers in the Epstein-Barr virus genome.
Virology
; 415(1): 1-5, 2011 Jun 20.
Article
en En
| MEDLINE
| ID: mdl-21550623
ABSTRACT
The human CCCTC-binding factor, CTCF, regulates transcription of the double-stranded DNA genomes of herpesviruses. The architectural complex cohesin and RNA Polymerase II also contribute to this organization. We profiled the occupancy of CTCF, cohesin, and RNA Polymerase II on the episomal genome of the Epstein-Barr virus in a cell culture model of latent infection. CTCF colocalizes with cohesin but not RNA Polymerase II. CTCF and cohesin bind specific sequences throughout the genome that are found not just proximal to the regulatory elements of latent genes, but also near lytic genes. In addition to tracking with known transcripts, RNA Polymerase II appears at two unannotated positions, one of which lies within the latent origin of replication. The widespread occupancy profile of each protein reveals binding near or at a myriad of regulatory elements and suggests context-dependent functions.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Represoras
/
ARN Polimerasa II
/
Cromatina
/
Proteínas Cromosómicas no Histona
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Herpesvirus Humano 4
/
Proteínas de Ciclo Celular
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Virology
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos