Purification of an endothelin receptor from human placenta.
Biochem Biophys Res Commun
; 167(1): 251-7, 1990 Feb 28.
Article
en En
| MEDLINE
| ID: mdl-2155613
ABSTRACT
We have identified an endothelin (ET) binding protein on the membranes of human placenta and purified it to homogeneity. It is a polypeptide with an apparent Mol. Wt. of 40,000 and is a major protein to be labeled by cross-linking with either 125I-ET-1, -2, or -3. Binding studies with Scatchard analysis indicated the presence of a single class, high-affinity binding site with Kds of 57 pM, 480 pM and 40 nM for 125I-labeled ET-1, ET-2 and ET-3, respectively. These results suggest that the 40K protein is a major ET receptor in placenta and, most likely, can bind differentially to ET-1, ET-2 and ET-3.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptidos
/
Placenta
/
Endotelio Vascular
/
Receptores de Superficie Celular
Tipo de estudio:
Prognostic_studies
Límite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1990
Tipo del documento:
Article
País de afiliación:
Japón