Alphavirus adsorption to mosquito cells as viewed by freeze fracture immunolabeling.
Virology
; 415(2): 132-40, 2011 Jul 05.
Article
en En
| MEDLINE
| ID: mdl-21561635
ABSTRACT
Sindbis Virus (SV), the prototype alphavirus in the family togaviridae, infects both mammalian and insect cells. The ability of SV to infect cells possessing significantly different biochemical environments suggests that there may be a common mode of entry into each cell type. Previous studies show that up to 4h post infection cells are permeable to small ions and alpha sarcin suggesting that the plasma membrane is compromised as infection takes place. Thin-section electron microscopy has also shown SV to bind to the plasma membrane and lose its electron dense core through a pore like structure developed upon interaction of the virus with the cell surface. Using freeze-fracture replicas, thin-sections and antibody labeling the data presented herein show virus associated with intramembrane particles on mosquito cells. These data suggest that the intramembrane particles associated with SV may be part of the pore structure consisting of virus proteins and cell receptor.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Inmunohistoquímica
/
Alphavirus
/
Técnica de Fractura por Congelación
/
Culicidae
Tipo de estudio:
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
Virology
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos