Comparison of three detergent-free protein extraction protocols for white adipose tissue.
Anal Biochem
; 415(2): 215-7, 2011 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-21565151
ABSTRACT
A comparative study of three detergent-free protein extraction protocols--a differential centrifugal fractionation, a delipidation protocol based on the Bligh and Dyer method, and the trifluoroethanol addition as cosolvent to an aqueous buffer--was performed on white adipose tissue. The performance of the protocols directly compatible with liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) was evaluated based on the total protein extraction yield and the protein recovery from different functional and cellular compartments. The most suitable method for the extraction of white adipose tissue proteins from a wide range of cellular and structural compartments was the delipidation protocol based on the Bligh and Dyer method.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas
/
Centrifugación
/
Tejido Adiposo Blanco
/
Fraccionamiento Químico
Límite:
Animals
Idioma:
En
Revista:
Anal Biochem
Año:
2011
Tipo del documento:
Article
País de afiliación:
Suiza