Genotyping of Mycoplasma hyopneumoniae in wild boar lung samples.
Vet Microbiol
; 152(1-2): 191-5, 2011 Aug 26.
Article
en En
| MEDLINE
| ID: mdl-21571452
ABSTRACT
Mycoplasma hyopneumoniae is the etiologic agent of enzootic pneumonia (EP), an important cause of disease-associated losses in swine production and a role of wild boar in recurrent infections can be supposed. Genotypes of M. hyopneumoniae from wild boar are unknown but could indicate its role as a potential reservoir. Therefore, 34 lung samples being PCR-positive for M. hyopneumoniae from wild boar from the Geneva region in Switzerland were assayed by genotyping using the p146 and multi-locus sequence typing (MLST) approaches and compared to data from outbreak cases from domestic swine in Switzerland. Successful genotyping was dependent on a sufficiently high concentration of M. hyopneumoniae DNA in the samples as assessed by different real-time PCR assays. The p146 genotyping was more successful with 24 samples (70.5%) being typeable whereas only 6 samples (17.6%) could be genotyped using the MLST approach. Variability of genotypes was high but identical types were found in geographically related animals. Genotypes from wild boar showed phylogenetic relatedness to those from domestic pigs but no matching types could be identified. Results show that direct genotyping from wild boar lung samples is possible and provides a promising approach to investigate future EP outbreak related samples from wild boar.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sus scrofa
/
Mycoplasma hyopneumoniae
/
Neumonía Porcina por Mycoplasma
/
Genotipo
/
Pulmón
Tipo de estudio:
Prognostic_studies
Límite:
Animals
País/Región como asunto:
Europa
Idioma:
En
Revista:
Vet Microbiol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Suiza