T cell receptor-bearing cells among rat intestinal intraepithelial lymphocytes are mainly alpha/beta+ and are thymus dependent.
Eur J Immunol
; 20(5): 1193-6, 1990 May.
Article
en En
| MEDLINE
| ID: mdl-2162777
ABSTRACT
Rearrangement of both the beta and gamma chain T cell receptor (TcR) genes was detected in intestinal intraepithelial lymphocytes (IEL) from normal euthymic rats. Flow cytometric analyses showed that about 73% of the IEL were CD3+ (1F4) and that 67% were TcR alpha/beta+ (R73). About 5% of the IEL were found to be CD3+, TcR alpha/beta- in double-labeling experiments suggesting that a small fraction of IEL in the rat express the alternative TcR gamma/delta. More than 70% of the IEL were granular implying that many CD3+ IEL are granular. In IEL from athymic nude rats no rearrangement of either the TcR beta or gamma chain genes or surface expression of CD3 or TcR alpha/beta was detected despite the fact that about 95% of the cells were granular and morphologically similar to those in normal rats. Taken together our data suggest that the majority of IEL in the rat express the conventional TcR alpha/beta and that TcR-bearing cells in the gut epithelium are thymus dependent.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Timo
/
Receptores de Antígenos de Linfocitos T
/
Linfocitos T
/
Mucosa Intestinal
Límite:
Animals
Idioma:
En
Revista:
Eur J Immunol
Año:
1990
Tipo del documento:
Article
País de afiliación:
Noruega