Production of γ-linolenic acid using a novel heterologous expression system in the oleaginous yeast Lipomyces kononenkoae.
Biotechnol Lett
; 33(10): 1993-8, 2011 Oct.
Article
en En
| MEDLINE
| ID: mdl-21681556
ABSTRACT
A novel expression system was established in the oleaginous yeast, Lipomyces kononenkoae. The expression vector pLK-rhPHG of L. kononenkoae was constructed and using the hygromycin phosphotransferase gene and green fluorescent protein gene as reporter genes. A delta 6-fatty acid desaturase gene (D6DM) from Cunninghamella echinulata MIAN6 was then expressed in this strain. The recombinant strain accumulated about 1.2% γ-linolenic acid in the total fatty acids.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Clonación Molecular
/
Ácido gammalinolénico
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Linoleoil-CoA Desaturasa
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Lipomyces
Idioma:
En
Revista:
Biotechnol Lett
Año:
2011
Tipo del documento:
Article