Yellow maize with high ß-carotene is an effective source of vitamin A in healthy Zimbabwean men.
Am J Clin Nutr
; 94(2): 510-9, 2011 Aug.
Article
en En
| MEDLINE
| ID: mdl-21715509
ABSTRACT
BACKGROUND:
The bioconversion efficiency of yellow maize ß-carotene to retinol in humans is unknown.OBJECTIVE:
The objective of this study was to determine the vitamin A value of yellow maize ß-carotene in humans.DESIGN:
High ß-carotene-containing yellow maize was grown in a hydroponic medium with 23 atom% (2)H(2)O during grain development. Yellow maize ß-carotene showed the highest abundance of enrichment as [(2)H(9)]ß-carotene. Eight healthy Zimbabwean men volunteered for the study. On day 1 after a fasting blood draw, subjects consumed 300 g yellow maize porridge containing 1.2 mg ß-carotene, 20 g butter, and a 0.5-g corn oil capsule. On day 8, fasting blood was drawn, and subjects consumed 1 mg [(13)C(10)]retinyl acetate in a 0.5-g corn oil capsule and 300 g white maize porridge with 20 g butter. Thirty-six blood samples were collected from each subject over 36 d. Concentrations and enrichments of retinol and ß-carotene in labeled doses and serum were determined with the use of HPLC, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry.RESULTS:
The area under the curve (AUC) of retinol from 1.2 mg yellow maize ß-carotene was 72.9 nmol · d, and the AUC of retinol from 1 mg retinyl acetate (13)C(10) was 161.1 nmol · d. The conversion factor of yellow maize ß-carotene to retinol by weight was 3.2 ± 1.5 to 1.CONCLUSION:
In 8 healthy Zimbabwean men, 300 g cooked yellow maize containing 1.2 mg ß-carotene that was consumed with 20.5 g fat showed the same vitamin A activity as 0.38 mg retinol and provided 40-50% of the adult vitamin A Recommended Dietary Allowance. This trial was registered at clinicaltrials.gov as NCT00636038.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vitamina A
/
Beta Caroteno
/
Zea mays
Tipo de estudio:
Prognostic_studies
Límite:
Adult
/
Humans
/
Male
/
Middle aged
País/Región como asunto:
Africa
Idioma:
En
Revista:
Am J Clin Nutr
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos