Cytotoxicity evaluation of Gutta Flow and Endo Sequence BC sealers.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
; 112(5): 657-61, 2011 Nov.
Article
en En
| MEDLINE
| ID: mdl-21778087
ABSTRACT
OBJECTIVE:
This study evaluated the cytotoxicity of GuttaFlow and EndoSequence BC sealers and compared them with AH Plus and Tubli-Seal sealers. STUDYDESIGN:
Samples (0.5 mg) of freshly mixed or set BC, GuttaFlow, AH Plus, and Tubli-Seal sealers were eluted with 300, 600, and 1,000 µL cell culture medium for 24 and 72 hours. L929 cells were seeded into 96-well plates at 3 × 10(4) cells/well and cultured with 100 µL eluate from each eluate group. Cells cultured only with culture medium served as control. After 24 hours' incubation, the cytotoxicity was evaluated by MTT assay. Cell viability was calculated as the percentage of the control group, and the results were analyzed with a one-way analysis of variance.RESULTS:
For the freshly mixed sealer, cell viability in the AH Plus group was less than in all of the other 3 sealer groups. The Tubli-Seal sealer group had less cell viability than the EndoSequence BC and GuttaFlow sealer groups. For the set sealer, the Tubli-Seal and AH Plus groups had less cell viability than the EndoSequence BC and GuttaFlow sealer groups. There was no cell viability difference between the EndoSequence BC and GuttaFlow sealer groups in the either freshly mixed or set sealer group.CONCLUSIONS:
The GuttaFlow and EndoSequence BC sealers have lower cytotoxicity than the AH Plus and Tubli-Seal sealers.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Materiales de Obturación del Conducto Radicular
/
Supervivencia Celular
/
Fibroblastos
Límite:
Animals
Idioma:
En
Revista:
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
Asunto de la revista:
ODONTOLOGIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos